Plasmid

Part:BBa_K4669030:Design

Designed by: Jonas Westphal   Group: iGEM23_Hamburg   (2023-10-12)


pET22b(+)_R12-ferritin


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5338
    Illegal SpeI site found at 33
    Illegal PstI site found at 1318
    Illegal PstI site found at 5675
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 33
    Illegal PstI site found at 1318
    Illegal PstI site found at 5675
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5272
    Illegal XhoI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5338
    Illegal SpeI site found at 33
    Illegal PstI site found at 1318
    Illegal PstI site found at 5675
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5338
    Illegal SpeI site found at 33
    Illegal PstI site found at 1318
    Illegal PstI site found at 5675
    Illegal NgoMIV site found at 316
    Illegal NgoMIV site found at 3492
    Illegal NgoMIV site found at 3652
    Illegal NgoMIV site found at 5240
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Coding sequences were codon optimized for E. coli.


Source

Synthetic

References